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Science:科学家们可以“看穿”这些老鼠

摘要 : 拥有透视眼看穿物品听起来就像是漫画书里才会有的故事,但研究人员已经找到一种方法可以把整个动物变透明——从它们的大脑到其骨头。

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拥有透视眼看穿物品听起来就像是漫画书里才会有的故事,但研究人员已经找到一种方法可以把整个动物变透明——从它们的大脑到其骨头。这种方法可以让荧光蛋白在整个身体里明显地发光,照亮整个血管系统(上图)和其他结构。为了创造出这种“灯光秀”,研究人员用几种有机溶剂处理了经过安乐死的小鼠,除去那些让它们不透明的水和脂质。该技术被称为uDISCO,因为它是原始DISCO技术的变种,DISCO是一种经溶剂清除的三维器官成像技术。虽然其他方法,如CLARITY或PARS,也可以把动物组织变透明,但uDISCO可以在短短几天而非几个星期或几个月就可以将整个动物身体变透明。这种技术可以实现最高分辨率的全身成像,这项技术的开发研究人员今天在《自然方法》网络版上报告了他们的成果。因为对小鼠实施解剖后几乎不可能再将其全身数百万计的神经元重新连接,因此该技术将有助于科学家更好地观察一种疾病是如何影响整个身体的神经元的,比如中风如何导致神经肌肉无力。同时,uDISCO并不仅仅限于神经系统的研究。它的应用几乎无止境,它可以赋予任何科学家看穿小鼠的“超能力”。

原文链接:

Scientists can see through these rodents

原文摘要:

Seeing through objects may seem like something straight out of a comic book, but researchers have found a way to make entire animals transparent—from their brains to their bones. The method lets fluorescent proteins visibly shine through bodies, lighting up entire vascular systems (above) and other structures. To produce such light shows, researchers treat euthanized rodents with several organic solvents to remove the water and lipids that made them opaque. The technique is dubbed uDISCO because it’s a variant of the original DISCO technique, which stands for 3D imaging of solvent-cleared organs. Although other methods like CLARITY or PARS can similarly create transparency in animal tissues, uDISCO does it for the whole body in just a few days as opposed to weeks or months. This technique allows the highest resolution images yet for a whole body, its creators report online today in Nature Methods. That should help scientists better see how a disease affects neurons throughout the body—like how a stroke can cause neuromuscular weakness—because it’s nearly impossible to reconnect the millions of neurons throughout a rodent body once dissected. uDISCO isn’t just limited to nervous system studies, either. Its applications are nearly endless, giving any scientist the seemingly superhuman ability to see through rodents.

来源: Science 浏览次数:0

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