Science

植物&动物 | 生态&环境 | 大脑&行为 | 健康 | 技术 | 科学&政策 | 进化 | 古生物学 | 细胞 | 分子 | 基因&蛋白 | 遗传&发育 | 生物化学 | 生物物理 | 免疫 | 人物&事件 | 微生物学 |
当前位置: Science » 遗传&发育 » Science:培养皿孵出的鼠卵

Science:培养皿孵出的鼠卵

摘要 : 这只老鼠也许看上去没什么特别,但它开创了先河。用来造出这只老鼠的卵细胞并不是来自一个成年母鼠的卵巢,而是在培养皿中从一个小鼠胎儿的卵原细胞培育而成。

 20160711.ScienceShots.large

这只老鼠也许看上去没什么特别,但它开创了先河。用来造出这只老鼠的卵细胞并不是来自一个成年母鼠的卵巢,而是在培养皿中从一个小鼠胎儿的卵原细胞培育而成。为了观察精子和卵细胞是如何形成的,研究人员长期以来一直在尝试通过诱导和培养让其成长的所谓原始生殖细胞(PGCs)来进行体外培育。(最近一个中国的研究小组报告说他们在试管中创造出了类精子细胞。)但直到现在,培养卵母细胞或是卵前体细胞都需要临时将原始生殖细胞和它们周围的细胞一起移植回老鼠的卵巢或肾脏里。在培养皿中,应该含有正在发育的卵子的卵泡通常会变形并挤在一起。最近发表在《美国科学院学报》网络版上的一项新研究提出了一个可能的原因:培养基中的雌激素过多。当研究人员用一种阻碍雌激素受体的复合物来培养小鼠卵巢胚胎时,其中的卵泡正常分离了。得到的卵子受精并被植入代孕母鼠后,产出了健康的幼鼠,比如图中的这只小鼠。这项新技术将允许研究人员通过观察卵母细胞与其周围指导其发育的细胞之间的复杂信号转导,更好地了解卵子如何成熟。有些人将这个成果看作是向更远大雄伟的目标迈进的一步:如果类似的技术可以对人类细胞起作用,那么某一天科学家们或许就能够利用女性体内的其他细胞,比如皮肤细胞,来创造健康的卵子,从而治疗不孕不育。

原文链接:

Mouse eggs grown in lab dish

原文摘要:

This mouse may not look like anything special, but it’s the first of its kind. The egg cell used to create the rodent didn’t come from the ovaries of an adult mother, but were developed in a dish from egg precursor cells of a mouse fetus. To observe how sperm and egg cells form, researchers have long been trying to grow them outside the body, by coaxing and culturing the so-called primordial germ cells (PGCs) that give rise to them. (A research team in China recently reported the creation of spermlike cells in vitro.) But until now, efforts to grow oocytes, or egg precursor cells, have involved temporarily grafting a mix of PGCs and their surrounding cells back onto a mouse ovary or kidney. In a dish, the follicles that are supposed to contain each developing egg typically end up malformed and clumped together. A new study published online today in the Proceedings of the National Academy of Sciences proposes a potential reason: too much estrogen in their culture medium. When the researchers cultured fetal mouse ovaries with a compound that blocks estrogen receptors, the follicles inside separated properly. And after being fertilized and implanted into surrogate mothers, the resulting eggs produced apparently healthy mice like the one above. The new technique will allow researchers to better understand how eggs mature by directly observing complex signaling between an oocyte and the surrounding cells that direct its development. And some see the work as a step toward a more distant and ambitious goal: If an analogous technique can work in human cells, scientists might someday treat infertility by making healthy eggs from other cells in a woman’s body, such as skin cells.

来源: Science 浏览次数:0

我们欢迎生命科学领域研究成果、行业信息、翻译原创、实验技术、采访约稿。-->投稿

RSS订阅 | 生物帮 | 粤ICP备11050685号-3 ©2011-2014 生物帮 Science  All rights reserved.